7NTO
The structure of RRM domain of human TRMT2A at 1.23 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-28 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000033 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 52.880, 36.300, 39.100 |
Unit cell angles | 90.00, 119.18, 90.00 |
Refinement procedure
Resolution | 34.140 - 1.230 |
R-factor | 0.1292 |
Rwork | 0.127 |
R-free | 0.17160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ntn |
RMSD bond length | 0.018 |
RMSD bond angle | 2.129 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.260 |
High resolution limit [Å] | 1.230 | 1.230 |
Number of reflections | 17520 | 1293 |
<I/σ(I)> | 18.2 | 1.95 |
Completeness [%] | 97.0 | |
Redundancy | 6.62 | |
CC(1/2) | 1.000 | 0.614 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M cesium sulfate and 2.2 M ammonium sulfate |