7NTN

The structure of RRM domain of human TRMT2A at 2 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.009300
Spacegroup name	F 4 3 2
Unit cell lengths	131.450, 131.450, 131.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.475 - 2.016
R-factor	0.2072
Rwork	0.205
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	sequence
RMSD bond length	0.006
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.016	2.016
Number of reflections	6872	1079
<I/σ(I)>	36.42	3.85
Completeness [%]	100.0
Redundancy	29.45
CC(1/2)	1.000	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	292	0.22M lithium sulfate, 0.1M sodium acetate (pH 4.5) and 26 % PEG 6000