7NTN
The structure of RRM domain of human TRMT2A at 2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.009300 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 131.450, 131.450, 131.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.475 - 2.016 |
R-factor | 0.2072 |
Rwork | 0.205 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | sequence |
RMSD bond length | 0.006 |
RMSD bond angle | 0.975 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.016 | 2.016 |
Number of reflections | 6872 | 1079 |
<I/σ(I)> | 36.42 | 3.85 |
Completeness [%] | 100.0 | |
Redundancy | 29.45 | |
CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 292 | 0.22M lithium sulfate, 0.1M sodium acetate (pH 4.5) and 26 % PEG 6000 |