7NTE
The structure of an open conformation of the SBP TarP_Csal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.770, 63.620, 73.700 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 53.430 - 1.600 |
| R-factor | 0.1778 |
| Rwork | 0.176 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ntd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.430 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 0.603 |
| Rmeas | 0.715 | |
| Rpim | 0.038 | 0.379 |
| Number of reflections | 74222 | 5395 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 98.2 | 96.8 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.998 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.2 M MgCl2, 0.1 M Hepes pH 7.0 and 25 % (w/v) PEG 6000 |
