7NTD
The structure of the SBP TarP_Csal in complex with ferulate
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 |
Unit cell lengths | 49.860, 52.280, 68.530 |
Unit cell angles | 101.68, 98.59, 104.90 |
Refinement procedure
Resolution | 47.124 - 1.750 |
Rwork | 0.191 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7nsw |
RMSD bond length | 0.007 |
RMSD bond angle | 1.458 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.890 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.080 | 0.687 |
Rpim | 0.073 | 0.620 |
Number of reflections | 62516 | 4572 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 96.8 | 95.4 |
Redundancy | 2 | 2 |
CC(1/2) | 0.988 | 0.470 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 0.2 M MgCl2, 0.1 M Tris pH 8.5 and 20 % (w/v) PEG 8000 |