7NRW
Human myelin protein P2 mutant M114T
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-03-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.790, 64.790, 100.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.720 - 2.000 |
Rwork | 0.207 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bvm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.118 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.255 | |
Number of reflections | 15156 | 1092 |
<I/σ(I)> | 11.59 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 13.8 | |
CC(1/2) | 0.994 | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.4 M Sodium malonate |