7NQF

Prim-Pol Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137 with dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 21 21 2
Unit cell lengths	77.041, 160.362, 39.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.440 - 2.020
R-factor	0.2144
Rwork	0.212
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nqd
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.180	2.060
High resolution limit [Å]	2.020	2.020
Rmerge	0.159	1.986
Rmeas	0.182	2.257
Rpim	0.086	1.059
Number of reflections	33089	1622
<I/σ(I)>	5.6	0.7
Completeness [%]	99.8	99.9
Redundancy	4.3	4.4
CC(1/2)	0.995	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	287	Sodium cacodylate PEG 4000 Sodium Chloride Spermine Cobalt chloride Guanosine triphosphate