7NQE

Prim-Pol Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137 with dGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	39.953, 62.675, 82.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.900 - 1.280
R-factor	0.1643
Rwork	0.163
R-free	0.19010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nqd
RMSD bond length	0.006
RMSD bond angle	1.007
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.680	1.300
High resolution limit [Å]	1.280	1.280
Rmerge	0.080	1.642
Rmeas	0.088	1.914
Rpim	0.036	0.956
Number of reflections	53746	2412
<I/σ(I)>	9.6	1
Completeness [%]	99.2	90.6
Redundancy	5.9	3.5
CC(1/2)	0.999	0.284

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0.2M Potassium Thiocyanate 20% PEG 3350 10mM MnCl2 2mM dGTP