7NQD

Prim-Pol Domain of CRISPR-associated Prim-Pol (CAPP) from Marinitoga sp. 1137

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-04
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	P 65
Unit cell lengths	97.380, 97.380, 109.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.790 - 2.970
R-factor	0.2172
Rwork	0.216
R-free	0.23990
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.620
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	SHELXDE
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.790	3.020
High resolution limit [Å]	2.970	2.970
Rmerge	0.219	1.752
Rmeas	0.237	1.896
Rpim	0.090	0.717
Number of reflections	12181	605
<I/σ(I)>	8.5
Completeness [%]	100.0	99.7
Redundancy	6.75	6.9
CC(1/2)	0.992	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	287	0.1M PCTP 25% PEG 1500