7NQ6

High resolution crystal structure of C-terminal domain (residues 715-866) of Nucleoporin-98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	72.070, 72.070, 91.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.560 - 1.500
R-factor	0.1722
Rwork	0.171
R-free	0.19190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e0q
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.600	1.540
High resolution limit [Å]	1.500	1.500
Rmeas	0.148	0.470
Rpim	0.015	1.100
Number of reflections	39371	2857
<I/σ(I)>	33.4	0.8
Completeness [%]	100.0	100
Redundancy	90.2	37.8
CC(1/2)	1.000	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	8% (v/w) PEG 6000, 8% (v/v) 2-methy-2,4-pentanediol, 0.1 M HEPES