7NPO

Branched K48-K63-Ub3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-28
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.873128
Spacegroup name	C 1 2 1
Unit cell lengths	59.630, 77.570, 50.610
Unit cell angles	90.00, 123.97, 90.00

Refinement procedure

Resolution	24.730 - 2.190
R-factor	0.2269
Rwork	0.224
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.003
RMSD bond angle	0.566
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.730	2.268
High resolution limit [Å]	2.190	2.190
Number of reflections	9868	1910
<I/σ(I)>	8.8	2.2
Completeness [%]	99.6
Redundancy	2	2
CC(1/2)	0.997	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M Ammonium acetate, 20 mM Tris pH 7.5, 50 mM NaCl, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v PEG 4000; 22 mg/ml