7NOW

Complex of Nucleoporin-98 and nanobody MS98-27 solved at 1.85A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	113.800, 149.810, 85.090
Unit cell angles	90.00, 133.09, 90.00

Refinement procedure

Resolution	47.830 - 1.850
R-factor	0.1851
Rwork	0.184
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e0q
RMSD bond length	0.007
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	1.900
High resolution limit [Å]	1.850	1.850
Rmeas	0.070
Rpim	0.029
Number of reflections	88309	6453
<I/σ(I)>	16.9	0.65
Completeness [%]	99.3	98.4
Redundancy	6.8	5.9
CC(1/2)	0.999	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	278	34% (v/v) PO/OH, 0.1 M Tris-HCl pH9, 0.16 M ammonium sulfate