7NMO

Crystal structure of beta-2-microglobulin D76A mutant

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	I 1 2 1
Unit cell lengths	55.665, 28.799, 62.289
Unit cell angles	90.00, 98.41, 90.00

Refinement procedure

Resolution	44.410 - 1.200
Rwork	0.130
R-free	0.15860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yxf
RMSD bond length	0.021
RMSD bond angle	2.188
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.410	1.220
High resolution limit [Å]	1.200	1.200
Rpim	0.014	0.066
Number of reflections	30020	1429
<I/σ(I)>	32.4	10.2
Completeness [%]	97.7	92.3
Redundancy	6.3	5.2
CC(1/2)	0.999	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	100 nL mother liquor / 200 nL protein 15% Glycerol, 0.1 M NaAcetate pH 4.5-5.5, 28-32%PEG 4000, 0.2 M NH4Acetate