7NMM

Complex of rice blast (Magnaporthe oryzae) effector protein APikL2F with the host target sHMA94 from Setaria italica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91188
Spacegroup name	C 2 2 21
Unit cell lengths	125.444, 222.479, 102.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.510 - 2.300
R-factor	0.2092
Rwork	0.207
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nlj
RMSD bond length	0.013
RMSD bond angle	1.612
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.510	2.360
High resolution limit [Å]	2.300	2.300
Rmeas	0.087
Number of reflections	63610	4432
<I/σ(I)>	10.6
Completeness [%]	99.9	99.8
Redundancy	6.6
CC(1/2)	0.999	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	0.2 M lithium sulfate, 0.1 M BIS-TRIS pH 6.5, 25 % PEG3350