Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 54.630, 28.871, 67.508 |
| Unit cell angles | 90.00, 102.02, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.200 |
| Rwork | 0.158 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.644 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rpim | 0.046 | 0.306 |
| Number of reflections | 31346 | 1236 |
| <I/σ(I)> | 12.5 | 3.5 |
| Completeness [%] | 96.3 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.994 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 100 nL mother liquor / 200 nL protein 15% Glycerol, 0.1 M NaAcetate pH 4.5-5.5, 28-32%PEG 4000, 0.2 M NH4Acetate |
