7NMA
Crystal structure of 14-3-3 sigma in complex with 13mer Amot-p130 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-12 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5419 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.250, 112.717, 62.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.960 - 1.750 |
R-factor | 0.1723 |
Rwork | 0.170 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jc3 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.927 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.960 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.119 | |
Number of reflections | 29576 | 1931 |
<I/σ(I)> | 10.2 | 2.8 |
Completeness [%] | 98.7 | |
Redundancy | 6 | |
CC(1/2) | 0.995 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.095 M Hepes pH 7.5, 26% PEG 400, 0.19 M CaCl2 and 5% Glycerol |