7NLI

S. cerevisiae Ty1 p22 restriction factor, Gag CA-CTD, AUG2 variant

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97928, 0.97943, 0.97281
Spacegroup name	P 65 2 2
Unit cell lengths	280.890, 280.890, 40.405
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	91.943 - 3.119
R-factor	0.2346
Rwork	0.233
R-free	0.25780
Structure solution method	MAD
Starting model (for MR)	Ty1 p22
Data reduction software	DIALS (1.6.3)
Data scaling software	Aimless (0.6.2)
Phasing software	SHELXDE (2018/1)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	140.450	3.830	3.330
High resolution limit [Å]	3.119	3.500	3.119
Rmeas	0.246	0.970	1.313
Rpim		0.177	0.167
Number of reflections	31760	2927	5059
<I/σ(I)>	24.5	5.2	6.8
Completeness [%]	100.0	100	100
Redundancy	112.8	56.7	115.8
CC(1/2)	0.983	1.000	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	260 nL Protein (13.6 mg/ml in 50 mM Sodium Acetate pH 5.0, 300 mM NaCl 1mM TCEP) 120 nL Mother Liquor (27.9% Glycerol, 17.7% PEG 4K 0.1M HEPES pH 7.5) 20 nL Seed solution (seeds in 20% Glycerol, 31% PEG 4K 0.1M HEPES pH 7.5)