7NLH

S. cerevisiae Ty1 p22 restriction factor, Gag CA-CTD, AUG1 variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97954
Spacegroup name	P 65 2 2
Unit cell lengths	282.124, 282.124, 39.967
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	141.460 - 2.800
R-factor	0.2568
Rwork	0.256
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ty1 p22 Aug2 variant partial model
RMSD bond length	0.004
RMSD bond angle	1.287
Data reduction software	DIALS (1.14.5)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	244.400	2.950
High resolution limit [Å]	2.800	2.800
Rmeas	0.101	3.912
Rpim	0.016	0.627
Number of reflections	23876	3390
<I/σ(I)>	21.6	1.2
Completeness [%]	100.0	100
Redundancy	38.7	38.6
CC(1/2)	1.000	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	300 nl protein (6.25 mg mL-1, in 20 mM Tris-HCl pH 8.5, 150 mM NaCl, 1 mM TCEP) 100 nl Mother Liquor (1.16 M Li2SO4, 0.1 M Tris-HCl pH 9.0) pH screened 7.5-9.0, Li2SO4 screened 1.125M-1.25M.