7NLF
Crystal structure of Mycobacterium tuberculosis ArgB in apo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9865 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 173.950, 173.950, 72.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.215 - 2.080 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ap9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.881 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (1.10) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 86.970 | 86.970 | 2.190 |
| High resolution limit [Å] | 2.075 | 6.560 | 2.080 |
| Rmerge | 0.047 | 0.024 | 0.922 |
| Rmeas | 0.050 | 0.026 | 0.969 |
| Rpim | 0.016 | 0.008 | 0.299 |
| Total number of observations | 7831 | 38396 | |
| Number of reflections | 25346 | 848 | 3662 |
| <I/σ(I)> | 22.2 | 59.8 | 2.3 |
| Completeness [%] | 100.0 | 99.8 | 99.9 |
| Redundancy | 10.1 | 9.2 | 10.5 |
| CC(1/2) | 1.000 | 1.000 | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.5 | 291 | 1260 mM ammonium sulphate 100 mM CHES pH 9.5 200 mM NaCl |
