7NKF

Hen egg white lysozyme (HEWL) Grown inside (Not centrifuged) HARE serial crystallography chip.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	78.800, 78.800, 38.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.720 - 1.700
R-factor	0.1766
Rwork	0.175
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dpx
RMSD bond length	0.013
RMSD bond angle	1.254
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.720	1.830
High resolution limit [Å]	1.700	1.700
Number of reflections	13921	1358
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	174
CC(1/2)	0.953	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	33% PEG 4000, 0.5 M NaCl, 50 mM sodium acetate pH 4.5