7NJI

HEX1 (in cellulo) loaded on HARE serial crystallography chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-05-01
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9762
Spacegroup name	P 65 2 2
Unit cell lengths	58.900, 58.900, 193.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.010 - 2.300
R-factor	0.2212
Rwork	0.219
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1khi
RMSD bond length	0.010
RMSD bond angle	1.157
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.940	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	9522	917
<I/σ(I)>	3.2
Completeness [%]	100.0
Redundancy	93
CC(1/2)	0.909	0.382

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	Grown inside Spodoptera frugiperda Sf9 insect cells