7NJH

HEX1 (in cellulo) grown inside HARE serial crystallography chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9762
Spacegroup name	P 65 2 2
Unit cell lengths	58.550, 58.550, 192.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.710 - 2.500
R-factor	0.2219
Rwork	0.220
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1khi
RMSD bond length	0.007
RMSD bond angle	0.878
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.100	50.710
High resolution limit [Å]	2.500	2.500
Number of reflections	7394	706
<I/σ(I)>	3.1
Completeness [%]	99.9
Redundancy	37
CC(1/2)	0.836	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	Transfected and grown in Spodoptera frugiperda Sf9 insect cells