7NJE

gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-06-27
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	38.400, 89.200, 111.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.600 - 3.000
R-factor	0.2367
Rwork	0.235
R-free	0.27110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fd8
RMSD bond length	0.004
RMSD bond angle	0.835
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.600	3.108
High resolution limit [Å]	3.000	3.000
Number of reflections	8132	787
<I/σ(I)>	2.5
Completeness [%]	100.0
Redundancy	48
CC(1/2)	0.957	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	50 mM HEPES pH 7.0, 20% PEG3350, 0.1 M sodium acetate pH 5.45