7NJE
gamma(S)-crystallin 9-site deamidation mutant grown inside HARE serial crystallography chip
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2020-06-27 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.400, 89.200, 111.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.600 - 3.000 |
R-factor | 0.2367 |
Rwork | 0.235 |
R-free | 0.27110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fd8 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.835 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.600 | 3.108 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 8132 | 787 |
<I/σ(I)> | 2.5 | |
Completeness [%] | 100.0 | |
Redundancy | 48 | |
CC(1/2) | 0.957 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 50 mM HEPES pH 7.0, 20% PEG3350, 0.1 M sodium acetate pH 5.45 |