7NHE
Crystal structure of Arabidopsis thaliana Pdx1K166R-I333 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.980023 |
Spacegroup name | H 3 |
Unit cell lengths | 177.973, 177.973, 115.121 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 92.230 - 2.230 |
R-factor | 0.1774 |
Rwork | 0.175 |
R-free | 0.21920 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lnu |
Data reduction software | DIALS |
Data scaling software | Aimless (0.5.17) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 92.230 | 92.230 | 2.280 |
High resolution limit [Å] | 2.230 | 10.440 | 2.230 |
Rmerge | 0.104 | 0.059 | 0.886 |
Rmeas | 0.120 | 0.070 | 1.040 |
Rpim | 0.059 | 0.035 | 0.534 |
Total number of observations | 260302 | 2697 | 17113 |
Number of reflections | 66508 | 640 | 4475 |
<I/σ(I)> | 7.2 | 16.7 | 2.4 |
Completeness [%] | 99.7 | 99.2 | 99.8 |
Redundancy | 3.9 | 4.2 | 3.8 |
CC(1/2) | 0.990 | 0.957 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 28.1% PEG 1000 (w/v) and 100 mM HEPES pH 7.0 |