7NH7

OC43 coronavirus methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-01
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5419
Spacegroup name	P 31
Unit cell lengths	61.945, 61.945, 109.717
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.350 - 2.200
R-factor	0.1665
Rwork	0.164
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yz1
RMSD bond length	0.005
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.350	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.131
Number of reflections	23828	2385
<I/σ(I)>	11.66	2.48
Completeness [%]	99.7
Redundancy	5.1
CC(1/2)	0.981	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	JSCG screen