7NG2
Crystal structure of Toxoplasma CPSF4-YTH domain in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 40.180, 40.180, 202.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.530 - 1.230 |
| R-factor | 0.1781 |
| Rwork | 0.177 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.771 |
| Data reduction software | PHENIX |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.530 | 1.274 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Number of reflections | 49444 | 4552 |
| <I/σ(I)> | 20.3 | 1 |
| Completeness [%] | 99.3 | 93.7 |
| Redundancy | 11 | 4.3 |
| CC(1/2) | 1.000 | 0.427 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20 % PEG 4000, 20% isopropanol and 0.1M tri-sodium citrate pH 5.6 |
