7NG0

Crystal structure of N- and C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-11
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	105.067, 106.562, 42.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.440 - 2.950
R-factor	0.2693
Rwork	0.267
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nfu
RMSD bond length	0.007
RMSD bond angle	1.331
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.440	37.440	3.130
High resolution limit [Å]	2.950	8.850	2.950
Rmerge	0.281	0.080	1.343
Rmeas	0.293	0.084	1.399
Rpim	0.082	0.025	0.388
Number of reflections	5285	229	835
<I/σ(I)>	5.5
Completeness [%]	100.0	99.3	100
Redundancy	12.7	10.7	12.7
CC(1/2)	0.997	1.000	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	NULL