7NG0
Crystal structure of N- and C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-11 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 105.067, 106.562, 42.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.440 - 2.950 |
R-factor | 0.2693 |
Rwork | 0.267 |
R-free | 0.28810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7nfu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.331 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.440 | 37.440 | 3.130 |
High resolution limit [Å] | 2.950 | 8.850 | 2.950 |
Rmerge | 0.281 | 0.080 | 1.343 |
Rmeas | 0.293 | 0.084 | 1.399 |
Rpim | 0.082 | 0.025 | 0.388 |
Number of reflections | 5285 | 229 | 835 |
<I/σ(I)> | 5.5 | ||
Completeness [%] | 100.0 | 99.3 | 100 |
Redundancy | 12.7 | 10.7 | 12.7 |
CC(1/2) | 0.997 | 1.000 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | NULL |