7NFU
Crystal structure of C-terminally truncated Geobacillus thermoleovorans nucleoid occlusion protein Noc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9119 |
Spacegroup name | P 21 3 |
Unit cell lengths | 146.790, 146.790, 146.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.890 - 2.500 |
R-factor | 0.2111 |
Rwork | 0.209 |
R-free | 0.23990 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.548 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 84.890 | 84.890 | 2.600 |
High resolution limit [Å] | 2.500 | 9.020 | 2.500 |
Rmerge | 0.091 | 0.024 | 2.654 |
Rmeas | 0.093 | 0.025 | 2.688 |
Rpim | 0.015 | 0.004 | 0.425 |
Number of reflections | 36704 | 866 | 4119 |
<I/σ(I)> | 28.5 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 39.7 | 35.1 | 39.7 |
CC(1/2) | 1.000 | 1.000 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | NULL |