7NF4
Structure of A. niger Fdc T395M R435P P438W variant (AnFdcII) in complex with prFMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-28 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.450, 97.010, 177.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.070 - 1.690 |
| R-factor | 0.19206 |
| Rwork | 0.191 |
| R-free | 0.21956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ev4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.663 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.070 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 124712 | 6130 |
| <I/σ(I)> | 7.8 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.5 |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | BIS-TRIS propane, potassium thiocyanate, PEG 3350 |
