7NF3
Structure of A. niger Fdc T395M variant (AnFdcI) in complex with prFMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.910, 63.850, 87.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.090 - 1.100 |
| R-factor | 0.15886 |
| Rwork | 0.158 |
| R-free | 0.17163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ev4 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.019 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.090 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 213550 | 8943 |
| <I/σ(I)> | 15.7 | 2.2 |
| Completeness [%] | 97.9 | |
| Redundancy | 4.9 | |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | BIS-TRIS propane, potassium thiocyanate, PEG 3350 |
