7NF0
T. atroviride Fdc variant TaFdcV in complex with hydroxylated prFMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.593, 74.593, 345.772 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.500 - 1.350 |
| R-factor | 0.16656 |
| Rwork | 0.166 |
| R-free | 0.18385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ev4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.923 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 245351 | 11383 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 8.8 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Calcium chloride dihydrate, sodium formate, PIPES, PEG smear high (BCS condition screen from Molecular Dimensions) |
