7NEY
Structure of T. atroviride Fdc wild-type (TaFdc) in complex with prFMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.212, 74.212, 346.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.190 - 1.740 |
| R-factor | 0.18376 |
| Rwork | 0.182 |
| R-free | 0.21499 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ev4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.731 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.190 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmeas | 0.185 | 0.833 |
| Number of reflections | 115391 | 5671 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 26.4 | 12.5 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | Ammonium acetate, sodium citrate, 15% PEG smear high (BCS screen from Molecular Dimensions) |
