7NDX
Crystal structure of the human HSP40 DNAJB1-CTDs in complex with a peptide of NudC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2020-02-24 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 41.300, 128.264, 135.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.530 - 2.541 |
Rwork | 0.233 |
R-free | 0.26810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3agy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.799 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.530 | 2.650 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.140 | |
Number of reflections | 12295 | 1465 |
<I/σ(I)> | 16.5 | |
Completeness [%] | 99.9 | |
Redundancy | 13 | |
CC(1/2) | 0.990 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 0.1 M of sodium acetate trihydrate and 15% (v/v) of polyethylene glycol 400 |