7NDJ

Crystal structure of ZC3H12C PIN-CCCH Zn Finger domain with RNA heptamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91841
Spacegroup name	P 41
Unit cell lengths	63.248, 63.248, 114.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.723 - 1.649
R-factor	0.1763
Rwork	0.175
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ndi
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.723	1.750
High resolution limit [Å]	1.649	1.649
Number of reflections	53908	8680
<I/σ(I)>	11.04
Completeness [%]	99.5
Redundancy	4.6
CC(1/2)	0.998	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	18% PEG 3350 and 0.2 M NaF pH 6.9