7NDH

Crystal structure of ZC3H12C PIN domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91841
Spacegroup name	P 41 21 2
Unit cell lengths	80.331, 80.331, 143.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.500 - 1.940
R-factor	0.1744
Rwork	0.172
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v34
RMSD bond length	0.015
RMSD bond angle	1.399
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	2.060
High resolution limit [Å]	1.940	1.940
Number of reflections	35462	3484
<I/σ(I)>	20.5
Completeness [%]	99.9	99.8
Redundancy	7.53
CC(1/2)	0.999	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M LiCl, 20% PEG 6000 and 0.1 M HEPES pH 7.0