7NCW
Crystal structure of oxidized glutaredoxin 2 from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION SUPERNOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-23 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.540562 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.383, 45.383, 104.238 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.300 - 2.400 |
| R-factor | 0.1986 |
| Rwork | 0.197 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fls |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.421 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.700 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.108 | 0.378 |
| Rmeas | 0.118 | 0.435 |
| Rpim | 0.046 | 0.206 |
| Number of reflections | 5212 | 723 |
| <I/σ(I)> | 12.3 | 3 |
| Completeness [%] | 99.1 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 277 | 30% (w/v) PEG 8000 0.1 M sodium cacodylate pH 6.5 0.2 M sodium acetate |
