7NCS

Lateral-open conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) bound by a bactericidal Fab fragment

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	7.1
Number of Particle	162844
Software	crYOLO,cryoSPARC,RELION,NAMD,MDFF,VMD,PHENIX,Coot,EPU,Gctf,UCSF Chimera
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION