7NC7

Crystal structure of fructose-bisphosphate aldolases FBAC from Bacillus methanolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-07-31
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	71.970, 98.260, 139.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.420 - 2.200
R-factor	0.1919
Rwork	0.189
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q94
RMSD bond length	0.007
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.420	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	24421	2158
<I/σ(I)>	6.7
Completeness [%]	99.8
Redundancy	4.1
CC(1/2)	0.996	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate trihydrate pH 4.5, 2.0 M ammonium sulfate