7NC1
Glutathione-S-transferase GliG with partially disordered active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.760, 79.760, 119.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.930 - 2.600 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.24570 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.479 |
| Number of reflections | 14009 | 1449 |
| <I/σ(I)> | 22.4 | 4.9 |
| Completeness [%] | 99.8 | |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 293 | 0.2 M Ammonium sulfate, 0.1 M Sodium acetate pH 4.7, 32 % PEG2000MME |
