7NBV
Structure of 2A protein from Theilers murine encephalomyelitis virus (TMEV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9159 |
Spacegroup name | P 21 3 |
Unit cell lengths | 73.788, 73.788, 73.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.600 - 1.870 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.23020 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.890 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | SHARP |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.590 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.113 | 1.191 |
Rpim | 0.026 | 0.273 |
Number of reflections | 22710 | 1644 |
<I/σ(I)> | 16.8 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19 | 19.8 |
CC(1/2) | 0.999 | 0.644 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 2A protein was concentrated to 4.38 mg/mL in 50 mM Tris (HCl) pH 7.4, 1.1 M NaCl, 1.0 mM DTT. 200 nL protein was mixed with 200 nL reservoir solution comprising 0.2 M KBr, 0.2 M potassium thiocyanate, 0.1 M sodium cacodylate pH 6.5, 3 % w/v poly-gamma-glutamic acid 200-400 and 10 % w/v PEG-MME 2000 |