7NBB
Branched Lys48- and Lys63-linked tri-ubiquitin (K48-K63-Ub3) in complex with synthetic nanobody NbSL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-29 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.87313 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.389, 97.615, 74.967 |
Unit cell angles | 90.00, 109.96, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 1.659 |
High resolution limit [Å] | 1.274 | 1.550 |
Rmerge | 0.099 | |
Number of reflections | 79157 | 3958 |
<I/σ(I)> | 8 | |
Completeness [%] | 85.3 | 34 |
Redundancy | 4 | 2.6 |
CC(1/2) | 0.992 | 0.296 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277.15 | 0.1 M Bis-Tris pH 7.2 0.28 M MgCl2 21% PEG3350 0.15 M NaCl 0.05 M Tris/HCl pH 7.5 |