7NAY
Crystal structure of lactate dehydrogenase from Selenomonas ruminantium with NADH.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Xenocs GeniX 3D Cu HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-12-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.750, 104.260, 110.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.610 - 1.840 |
| R-factor | 0.149 |
| Rwork | 0.145 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d0o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.596 |
| Data reduction software | XDS (20200417) |
| Data scaling software | XDS (20200417) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.610 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 31954 | 5035 |
| <I/σ(I)> | 12.17 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.6 | 5.3 |
| CC(1/2) | 0.990 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 21 % PEG 1500 150 mM Malonate-Imidazole-Boric Acid pH 5 (MIB-PACT) 10 mM Sodium Bicarbonate pH 8 10 mM Tb-XO4 |
