7N9L
KirBac3.1 C71S C262S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 106.884, 106.884, 89.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.800 - 2.400 |
R-factor | 0.2293 |
Rwork | 0.228 |
R-free | 0.25780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xl4 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.800 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.245 | 2.534 |
Number of reflections | 20993 | 3316 |
<I/σ(I)> | 8.6 | 0.52 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 13.16 | 11.84 |
CC(1/2) | 0.998 | 0.404 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 90 mM HEPES pH 7.5, 2.5 w/v % PEG 8K, 2.5% w/v PEG 4K, 20.9% w/w PEG 400, 10% v/v glycerol. |