7N9K
KirBac3.1 L124M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 106.577, 106.577, 89.597 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.080 - 2.720 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xl4 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.663 | 2.890 |
High resolution limit [Å] | 2.710 | 2.710 |
Rmeas | 0.170 | 1.410 |
Number of reflections | 14347 | 4231 |
<I/σ(I)> | 10.52 | 1.35 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 7.22 | |
CC(1/2) | 1.000 | 0.579 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 90 mM HEPES pH 7.5, 2.5 w/v % PEG 8K, 2.5% w/v PEG 4K, 20.9% w/w PEG 400, 10% v/v glycerol. |