7N9D
I74A mutant of the isopentenyl phosphate kinase from Candidatus methanomethylophilus alvus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-24 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 172.293, 172.293, 80.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.060 - 2.100 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lnv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.645 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.097 | 0.745 |
| Number of reflections | 41416 | 1983 |
| <I/σ(I)> | 12.32 | 1.189 |
| Completeness [%] | 99.4 | |
| Redundancy | 10.8 | |
| CC(1/2) | 0.992 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 273 | 3.5 M Sodium Formate pH 7.0 |
