7N91
P70 S6K1 IN COMPLEX WITH MSC2317067A-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-25 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.300, 74.550, 150.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.520 - 3.000 |
| Rwork | 0.212 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a60 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 3.170 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.148 | 0.522 |
| Rmeas | 0.168 | 0.564 |
| Number of reflections | 13883 | 2106 |
| <I/σ(I)> | 11.5 | 2.86 |
| Completeness [%] | 96.1 | 95.8 |
| Redundancy | 5.87 | 5.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2M Bis-Tris pH 5.5, 0.1M Li2SO4, 26-28% PEG3350 |
