7N8U
Crystal structure of Triosephosphate isomerase from Candidatus Prometheoarchaeum syntrophicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-09-30 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.95372 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.289, 121.350, 129.263 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.240 - 1.980 |
R-factor | 0.1678 |
Rwork | 0.166 |
R-free | 0.20670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b9b |
RMSD bond length | 0.007 |
RMSD bond angle | 0.862 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.240 | 2.100 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 43128 | 6790 |
<I/σ(I)> | 20.1 | |
Completeness [%] | 99.8 | |
Redundancy | 13.5 | |
CC(1/2) | 0.999 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 25 % v/v Polyethylene glycol monomethyl ether 550, 50 mM HEPES; pH 7.0, 10 mM Magnesium chloride |