7N8D
Crystal structure of R22A human Galectin-7 mutant in presence of Lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.530, 77.620, 111.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.890 - 2.490 |
R-factor | 0.2443 |
Rwork | 0.242 |
R-free | 0.28130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gal |
RMSD bond length | 0.009 |
RMSD bond angle | 1.004 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.12) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.400 | 45.410 | 2.580 |
High resolution limit [Å] | 2.490 | 5.360 | 2.490 |
Rmerge | 0.068 | 0.034 | 0.194 |
Rmeas | 0.073 | 0.036 | 0.206 |
Rpim | 0.025 | 0.012 | 0.066 |
Number of reflections | 9873 | 1096 | 968 |
<I/σ(I)> | 22.2 | 32.4 | 11.4 |
Completeness [%] | 99.6 | 99.7 | 100 |
Redundancy | 8.9 | 8.4 | 9.6 |
CC(1/2) | 0.999 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295.15 | 0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 7.5 mM alpha Lactose |