7N8D

Crystal structure of R22A human Galectin-7 mutant in presence of Lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-19
Detector	RAYONIX MX300HE
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	30.530, 77.620, 111.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.890 - 2.490
R-factor	0.2443
Rwork	0.242
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gal
RMSD bond length	0.009
RMSD bond angle	1.004
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.400	45.410	2.580
High resolution limit [Å]	2.490	5.360	2.490
Rmerge	0.068	0.034	0.194
Rmeas	0.073	0.036	0.206
Rpim	0.025	0.012	0.066
Number of reflections	9873	1096	968
<I/σ(I)>	22.2	32.4	11.4
Completeness [%]	99.6	99.7	100
Redundancy	8.9	8.4	9.6
CC(1/2)		0.999	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295.15	0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000, 7.5 mM alpha Lactose