7N6K
Crystal structure of the substrate-binding domain of E. coli DnaK in complex with the peptide RALALLPLSR
Replaces: 7JMLExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-10 |
Detector | DECTRIS PILATUS3 R 200K-A |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 37.763, 94.773, 117.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.520 - 2.550 |
R-factor | 0.2128 |
Rwork | 0.208 |
R-free | 0.30650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dkz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.563 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.580 |
High resolution limit [Å] | 2.540 | 6.890 | 2.540 |
Rmerge | 0.161 | 0.084 | |
Rmeas | 0.185 | 0.102 | |
Rpim | 0.117 | 0.057 | |
Total number of observations | 21502 | ||
Number of reflections | 6943 | 376 | 313 |
<I/σ(I)> | 4 | ||
Completeness [%] | 96.6 | 91.3 | 89.9 |
Redundancy | 3.1 | 2.6 | 2.7 |
CC(1/2) | 0.986 | 0.307 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 2.6 M (NH4)2SO4, 0.1 M K3PO4 |