7N6H
The crystal structure of the GH30 subfamily 10 enzyme, AcXbh30A from Acetivibrio clariflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.868, 108.018, 66.836 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 41.180 - 1.280 |
| R-factor | 0.2098 |
| Rwork | 0.202 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6m5z |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.392 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.110 | 1.010 |
| Rmeas | 0.128 | 1.215 |
| Rpim | 0.064 | 0.642 |
| Number of reflections | 211708 | 8265 |
| <I/σ(I)> | 20 | 1.05 |
| Completeness [%] | 96.9 | 95.3 |
| Redundancy | 3.7 | 3.3 |
| CC(1/2) | 0.987 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 289 | 0.1M Sodium Acetate, 25% (w/v) PEG 4000 |
