7N5U

ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing GGACCC (Oligo 21)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	63.380, 103.606, 37.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.110 - 2.860
R-factor	0.2529
Rwork	0.248
R-free	0.29960
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.537
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.110	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.068	1.060
Rpim	0.020	0.377
Number of reflections	4830	162
<I/σ(I)>	25.3	0.9
Completeness [%]	79.5	22.6
Redundancy	11.3	6.6
CC(1/2)		0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	289	4% Tacsimate, pH 5.0, 12% PEG3350